http://rdf.ncbi.nlm.nih.gov/pubchem/patent/KR-100372777-B1
Outgoing Links
| Predicate | Object |
|---|---|
| classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A41B2400-52 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A41B9-002 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A41B9-001 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-54 |
| filingDate | 2000-06-17-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2003-02-19-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2003-02-19-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | KR-100372777-B1 |
| titleOfInvention | Thermostable DNA Polymerase-encoding Gene from Aquifex pyrophilus and Amino Acid Sequence Deduced Therefrom |
| abstract | The present invention relates to genes of heat resistant DNA polymerase isolated from Aquifex pyrophilus and amino acid sequences inferred therefrom. The inventors have used the Aquifex aeolicus DNA polymerase gene as a probe to include a gene for heat-resistant DNA polymerase ( Aquifex pyrophilus DNA polymerase, Apy DNA polymerase) from genomic DNA of Aquifex aeolicus . Sections were selected. Electrical segment Localization of hybridization was cloned into the E. coli colonies hive method Apy DNA polymerase enzyme screening the positive clones containing the gene fragment, and then, by separating the Apy DNA polymerase gene fragments from the clones found to be positive electropositive clones Apy It was reconfirmed that the DNA polymerase gene fragment was included. Subsequently, the nucleotide sequences of the Apy DNA polymerase gene fragments were determined and the amino acid sequence was inferred from the nucleotide sequence. The Apy DNA polymerase gene is composed of 1725 bp including an end codon and encodes a protein consisting of 574 amino acids. Was confirmed. Apy conventional heat-resistant DNA polymerase, the amino acid sequence of a DNA polymerase enzyme and E. coli DNA polymerase amino acid sequence and comparative analysis results of the I, Apy DNA polymerase of the present invention is a high sequence homology to conventional DNA polymerase The eggplant was identified as a new heat-resistant DNA polymerase, and in addition to its DNA polymerization activity, it was also estimated to have a proof-reading activity that improves the accuracy of DNA polymerization. The Apy DNA polymerase gene of the present invention is a high-efficiency, high-functional recombinant heat-resistant DNA polymerase, which has a higher function than the original enzyme by industrially mass-producing a heat-resistant DNA polymerase or using a protein engineering method through genetic engineering. It can be useful for production. |
| priorityDate | 2000-06-17-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 151.