http://rdf.ncbi.nlm.nih.gov/pubchem/patent/KR-100347870-B1
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_a3b2c46de145f3a764f36a1cd2ebdcf0 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/B29C66-5229 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-63 |
| filingDate | 1999-06-12-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2002-08-07-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_58b910d207c39e14d92cf274cb082f00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_86643027f29ba19e1272fa7c8bf06b4f |
| publicationDate | 2002-08-07-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | KR-100347870-B1 |
| titleOfInvention | Escherichia coli with plasmid pALN69 which was constructed by connecting with pKAL24 and gilthead seabeam growth hormone |
| abstract | The present invention to prepare a vector for high expression of growth hormonen n n PCR amplification of only growth hormone cDNA sites using primers OI and O-II in pGSBGH3, a vector containing a Gheadhead seabream growth hormone, and inserting the restriction enzyme Sal I and Hind III recognition sites into the socks and,n n n As a promoter for expressing the growth hormone, restriction enzymes Hind III, Sal I fragments of the promoter region of the marine microorganism Pseudomonas sp. Alginate lyase are used.n n n The isolated cDNA fragment and promoter fragment were ligation using T 4 ligase, and then amplified ligation fragment using primers OI and O-II and recognition of restriction enzyme Eco RI at the beginning of the promoter. A method of preparing a growth hormone high expression vector (pALN69) by inserting a site and ligation to a cloning vector pUC19. |
| priorityDate | 1999-06-12-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 157.