http://rdf.ncbi.nlm.nih.gov/pubchem/patent/KR-100344090-B1
Outgoing Links
| Predicate | Object |
|---|---|
| classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q2600-142 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-68 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-85 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-63 |
| filingDate | 2000-05-29-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2002-07-24-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2002-07-24-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | KR-100344090-B1 |
| titleOfInvention | Recombinant plasmid for toxicity test and a transformed cell line by that plasmid |
| abstract | The present invention relates to the production of a recombinant plasmid capable of containing enzymes involved in one plasmid and the cell line transformed with the plasmid in metabolic activation, which appears when a carcinogen or toxic substance is administered to the body. That is, the present invention is a multiple protein expression system, and more specifically, protein cytochrome p450 under one common promoter using an internal ribosome entry site in one vector. The present invention relates to a recombinant plasmid capable of simultaneously expressing 1A2 and its supporter NADPH-cytochrome reductase. In addition, the plasmid gene was transferred to Chinese hamster ovary (CHO) cells to establish a toxicity test model for in vitro metabolic activation. Furthermore, the present invention can be widely used in the study of cytochrome mechanisms, as well as in the study of autoimmune diseases, drug hypersensitivity reactions, neurological diseases and pharmaceutical preparation thereof. |
| priorityDate | 2000-05-29-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 928.