http://rdf.ncbi.nlm.nih.gov/pubchem/patent/KR-100342438-B1
Outgoing Links
| Predicate | Object |
|---|---|
| classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A01K67-0275 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-8771 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A01K67-0273 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N5-16 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N5-16 |
| filingDate | 1999-07-31-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2002-07-04-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2002-07-04-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | KR-100342438-B1 |
| titleOfInvention | Method for activation of fused cell for producing a clone animal using somatic cell |
| abstract | The method for activating a fusion cell of the present invention comprises the steps of: (a) collecting and culturing immature eggs from slaughter ovaries; (b) isolating and preserving cell lines from various tissues of the bovine body; (c) dissecting a portion of the zona pellucida of the mature egg prepared in step (a) and denuclearizing by removing 10-30% of the cytoplasm; (d) transplanting the donor cells prepared in step (b) into denucleated eggs of step (c); (e) electrically fusion of the embryonated eggs implanted and adhered in step (d) to a voltage under DC conditions; (f) activating the fused cells concurrently with fusion or in a medium to which drug is added; And (g) culturing the activated cells in post-activation and micro-media, wherein in the production of cloned cows, in the case of f), in the case of inducing activation by an electrofusion method, When electrofusion is carried out in a 2+ addition fusion medium and activation is induced by ionomycin treatment, it is allowed to stand for 4 minutes in 50 µl drops under shading conditions and 10% FBS or 30 mg / ml in a 35 mm dish. After inomycin is removed under a washing medium added with BSA, it is allowed to stand for 5 minutes. In addition, the post-activation step may be performed by immersing 5-10 eggs in 25 μl of cycloheximide for 6 hours, or culturing 5-10 eggs in 25 μl of DMAP for 4 hours. have. |
| priorityDate | 1999-06-30-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 145.