| abstract |
The present invention is to prepare a rare anti-cancer saponin by hydrolysis of ginseng saponin ginseng using a saponin glucoside degrading enzyme.n n n Common cellulose enzyme (cellulase) and hemi-cellulose enzyme (Hemi-cellulase) can hydrolyze the polysaccharide pull (多糖 體 糖 基), but it is difficult to break the saponin pull (糖 基). The reason is that saponins have non-glycol aglycone groups, so only special saponin degrading enzymes can decompose saponins. In the present invention, saponin degrading enzyme, for example, saponin alpha-glucosidase (alpha-glucosidase) to decompose the saponin suffix of the protopanaxadiol-type ginsenosides (high base) Ginsenoside Rh2 can be obtained with a yield of 40-80%.n n n In addition, ginsenoside Rg1 may be prepared by decomposing ginsenoside Rg2 succinic acid with alpha-rhamnosidase (also, ginsenoside Rg1 may be prepared by hydrolyzing ginsenoside Re). Yield is 40-80%. In addition, the mixed ginseng saponin is reacted with a saponin degrading enzyme, thereby preparing a mixed saponin having a high content of anti-cancer saponins ginsenosides Rh2 and Rh1. In addition, the ginseng that produces saponin-degrading enzymes can be cultured in ginseng to make ginseng powder, ginseng fragments and other products with high content of anti-cancer saponins ginsenosides Rh2 and Rh1. |