http://rdf.ncbi.nlm.nih.gov/pubchem/patent/KR-100309944-B1
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_167d2c5cfd906844123754cc069dc23f |
| classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q2600-124 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6834 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-689 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-68 |
| filingDate | 1999-06-17-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2001-11-01-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_e04b3d6efee94426dceb8c5142495904 |
| publicationDate | 2001-11-01-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | KR-100309944-B1 |
| titleOfInvention | Development of In situ Hybridization to detect Mycoplasma hyopneumoniae in formalin-fixed paraffin-embedded tissues without bacterial isolation |
| abstract | The present invention relates to a method for diagnosing epidemic pneumonia in swine using intracellular hybridization, and more specifically, a specific DNA probe of mycoplasma hyopneumoniae, a pathogen of swine enzootic pneumonia (SEP). The present invention relates to a method for diagnosing a pandemic pneumonia in a pig that diagnoses infection and distribution of pathogens quickly and without bacterial separation by using in situ hybridization. . Therefore, according to the present invention, the present invention was able to overcome the problem of the fluorescent antibody method using only frozen tissues because it uses the lung tissue fixed in formalin, and also can detect a gene specific to mycoplasma hyopneumoniae only. As a result, cross-reactions were able to overcome the problems of immunohistochemistry, which was somewhat less specific. In addition, it solved the problem of necessarily requiring fresh tissue while showing sensitivity as high as that of the gene-level method using the polymerase chain reaction, and also observed the cells infected with mycoplasma hyopneumoniae through the optical microscope. It can be quickly and easily diagnose the infection and distribution of the pathogen is effective. |
| priorityDate | 1999-06-17-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 55.