http://rdf.ncbi.nlm.nih.gov/pubchem/patent/KR-100299752-B1
Outgoing Links
| Predicate | Object |
|---|---|
| classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12R2001-84 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N5-16 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K14-815 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N1-165 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-80 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-12 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N5-10 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N5-00 |
| filingDate | 1998-07-07-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2001-10-27-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2001-10-27-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | KR-100299752-B1 |
| titleOfInvention | Elastase inhibitor genes isolated from Korean blood-sucking leeches, recombinant vectors and transformants containing them |
| abstract | The present invention relates to a gene encoding Guamerin, an elastase inhibitor isolated from Korean blood-sucking leech, an expression vector containing the same, and a yeast transformed therefrom, and more specifically, to isolate whole mRNA of leech. The cDNA library was constructed, and genes encoding the germins were isolated from the genes, the nucleotide sequences were identified, and the expression vector in yeast was constructed to express the genes using the genes. An expression vector prepared by linking a gene of a Germerine modified by PCR to help precise cleavage to a gene of an α-factor signal peptide that is induced outward, and inserted into a chromosome of Pichia pastoris , a methanol magnetizing yeast. Elastase by inducing expression by culturing in a medium where methanol is the only carbon source Since it was possible to prepare a large amount of the preparation, the elastase inhibitor prepared from the expression vector of the present invention is prepared in high yield by methanol, and the prepared protein is precisely processed to have the activity and enzyme specificity of the native germerin. Have |
| priorityDate | 1998-07-07-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 141.