http://rdf.ncbi.nlm.nih.gov/pubchem/patent/KR-100289500-B1
Outgoing Links
| Predicate | Object |
|---|---|
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N11-14 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N11-00 |
| filingDate | 1999-04-13-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2001-05-02-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2001-05-02-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | KR-100289500-B1 |
| titleOfInvention | Process for Preparing Immobilized Urokinase by Semi-covalent Binding |
| abstract | The present invention relates to a method for preparing immobilized urokinase for economically obtaining a target protein from a recombinant protein expressed in the form of a fusion protein that can be cleaved by urokinase. In the method for preparing immobilized urokinase of the present invention, the step of adding urokinase to a hydroxylapatite resin suspended in a buffer solution and stirring, adding bovine serum albumin (BSA) to the hydroxykinatite-adsorbed hydroxylapatite resin obtained above And stirring, and then, washing with a buffer solution, adding glutaraldehyde to a hydroxyl apatite resin adsorbed with urokinase and bovine serum albumin obtained in the past, stirring, and washing with a buffer solution. It is a manufacturing method. According to the present invention, the expensive urokinase can be economically cleaved and separated from the fusion protein by appropriately reusing the expensive urokinase, and thus it can be widely applied to the production of recombinant proteins in the form of other fusion proteins having a urokinase cleavage site. There will be. |
| priorityDate | 1999-04-13-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 51.