http://rdf.ncbi.nlm.nih.gov/pubchem/patent/KR-100274280-B1
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_5bc2b1e5b4536ecb82e63f40a57f2d6d http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_b112272aedc4e86b73610b0169c5cecf |
| classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-70 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12P17-04 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N9-0004 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-70 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-53 |
| filingDate | 1998-02-16-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2000-12-15-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_4f62580b2fa02b61390289a00e4a3663 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_a1766685788f61e89c2dcccbdaf1078c http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_23c90b25e0e018b7dd7ed413e0be9b61 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_25db31da24ecf639146cfdc7c5b0a6e8 |
| publicationDate | 2000-12-15-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | KR-100274280-B1 |
| titleOfInvention | Recombinant plasmids containing the D-arabino-1,4-lactone oxidase gene, transformants and methods for mass production of D-erythroascolic acid and L-ascolic acid using the same |
| abstract | The product of two parts of D-arabino-1,4-lactone oxidase, which is involved in D-erythroaskolbinic acid biosynthesis in Saccharomyces cerevisiae synthesized by gene amplification using primers, was introduced into pGEM-T Easy vector. Intact D-arabino-1,4-lactone oxidase was obtained through Sac I, a restriction enzyme within. They were overexpressed by the expression vector pET-3a from E. coli, the most well-known expression system of prokaryotes. Among them, remarkable enzymatic activity was observed in the water-soluble portion of D-arabino-1,4-lactone oxidase. It can be used to produce excess (or large quantities) of D-erythroascorbic acid and L-ascorbic acid. |
| priorityDate | 1998-02-16-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 272.