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classificationCPCAdditional http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A23V2300-31
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filingDate 1999-04-21-04:00^^<http://www.w3.org/2001/XMLSchema#date>
grantDate 2000-09-15-04:00^^<http://www.w3.org/2001/XMLSchema#date>
inventor http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_33df030653ac2edfa09348583bebe719
publicationDate 2000-09-15-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationNumber KR-100268603-B1
titleOfInvention Process for preparing mycelium extract
abstract In the first extraction method of the useful component from the mycelium-containing medium according to the present invention, a solid medium containing the mycelium obtained by inoculating basidiomycete on a solid medium based on bagasse and then propagating the mycelium is compressed. To obtain a juice solution and add water and an enzyme, preferably water and a mycelium cell wall lytic enzyme, to the solid residue component containing the mycelium from which the juice solution was separated and stirred while maintaining the temperature at 30 to 60 ° C. And dissolve the mycelium cell wall, and then heat to 95 ° C to deactivate and sterilize the enzyme to obtain cell wall lysis product-containing solution (ii).n n n In the second method according to the present invention, the solid residue component containing the mycelium obtained by separating as described above is once decanted, and then water and enzyme, preferably water and mycelium cell wall lytic enzyme, are added at this temperature. The solid component is ground and ground to dissolve the mycelium cell wall, and then enzyme deactivation and sterilization are performed as described above.n n n In the third method according to the present invention, the solid medium containing the mycelium obtained in the same manner as described above is discontinued, and the released solid medium is β-1,3-glucanase-containing liquid (a), preferably β-1. The mycelium cell wall was lysed by contacting the enzyme solution (a) containing chitinase and cellulase at a temperature of 30 to 60 ° C for 1 to 65 minutes with the main component of, 3-glucanase as the main component, and then the obtained cell wall lysis product-containing solution. It is characterized by inactivation and sterilization of the enzyme by heating to 95 ℃.n n n According to the present invention, useful components can be obtained in a high yield in a short time from the mycelium-containing medium. In particular, according to the first method, an extract containing β to glucan at a high concentration and excellent in antitumor effect is obtained. The juice component contains a high concentration of cytokinin-like substances and the like as well as metabolic components of the mycelium, and is expected to be used as a drink, plant hormone, and cosmetic raw materials.n n n In particular, according to the third method, an extract containing high concentrations of cytokinin-like substances and the like in addition to β-glucan and glucose is obtained, and thus it is expected to be used for the above uses.
isCitedBy http://rdf.ncbi.nlm.nih.gov/pubchem/patent/KR-20170024668-A
priorityDate 1995-12-25-04:00^^<http://www.w3.org/2001/XMLSchema#date>
type http://data.epo.org/linked-data/def/patent/Publication

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