http://rdf.ncbi.nlm.nih.gov/pubchem/patent/KR-100255269-B1
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_e82fc03c0fdcb1dadfc495c9ba125560 |
| classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2500-05 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-70 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N1-00 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N1-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-70 |
| filingDate | 1998-02-09-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2000-05-01-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_0b16aa5928e2ecaeb77668534a07ae1a http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_3fb8fb2c6c6a7bda2c9cbcbd876e3179 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_170bb1582e8b50356aed4f93f135e783 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_a0c7fe340d842b2cb0b276b44f565c32 |
| publicationDate | 2000-05-01-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | KR-100255269-B1 |
| titleOfInvention | DOSTAT fed-batch culture method for mass production of human growth hormone |
| abstract | The present invention relates to a dissolved oxygen (DO) -stat fed-batch culture method for mass production of human growth hormone (hGH). More specifically, the present invention is a batch culture of recombinant E. coli containing an inducible hGH fusion protein expression vector using L- arabinose operon of Salmonella strains from the initial culture medium, since the amount of dissolved oxygen rapidly increases The present invention relates to a DO-stat fed-batch cultivation method for hGH mass production, which includes culturing in fed medium and simultaneously adding L-arabinose at the beginning of fed-batch culture. According to the DO-stat fed-batch fermentation method of the present invention, the glycerol is used as the carbon source in the batch fermentation section to reduce the inhibitory effect of arabinose operon and the accumulation of acetic acid, and the carbon source in the oil price section using the amount of dissolved oxygen as an indicator. As a result, the problem of inhibiting hGH fusion protein expression was solved by maintaining glucose which is inexpensive and has excellent utilization efficiency below the expression inhibition minimum concentration. In addition, high concentrations of cell cultures and high levels of expression were obtained through oxygen addition. In addition, L-arabinose is added at the beginning of the incubation to maintain cell growth, plasmid stability, and expression level of the hGH fusion protein at a high level, thereby producing a large amount of hGH at a low production cost. |
| priorityDate | 1998-02-09-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 150.