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filingDate 2018-06-07-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationDate 2020-01-09-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationNumber JP-WO2018225835-A1
titleOfInvention Method for producing cell laminate
abstract A container having a bottom portion and a side wall portion rising from the outer edge of the bottom portion, a porous membrane, and a holding member for holding the porous membrane facing the inner bottom surface at a position not in contact with the inner bottom surface of the container, A method for producing a cell laminate having a cell layer on both surfaces, wherein a porous membrane is held by a holding member at a position not in contact with the inner bottom surface of the container so as to face the inner bottom surface, and the bottom of the container in the direction of gravity. Culturing the first cells in a liquid medium in contact with the inner bottom surface of the container and the surface of the porous membrane, with the porous membrane being at the top and the porous membrane at the bottom; The first cell is cultured on the lower surface of the porous membrane, in a state in which the bottom of the container is downward and the porous membrane is upward in the direction of gravity, and the first cell is cultured, Culture the second cell on the upper surface of the porous membrane When; including, manufacturing method of the cell stack.
priorityDate 2017-06-09-04:00^^<http://www.w3.org/2001/XMLSchema#date>
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