| abstract |
A method and apparatus for forming clusters of amplified nucleic acid fragments in an orderly arrangement on a substrate without amplification bias. In the method according to the present invention, on a substrate having a plurality of first surfaces having hydrophilicity, and a second surface surrounding each of the plurality of first surfaces, the second surface having a lower hydrophilicity than the first surface. Thus, a droplet in which a nucleic acid serving as a template is encapsulated is formed, a nucleic acid amplification reaction is performed within the droplet on the substrate, and then the droplet is removed and the nucleic acid amplification reaction is further performed on the substrate. |