| abstract |
A step of obtaining a 3D organoid by three-dimensionally culturing a tissue comprising at least one of human intestinal epithelial stem cells, human intestinal epithelial cells, or these cells on an extracellular matrix; The 3D organoid obtained in step 1 is dispersed to prepare single cells, and the single cells are cultured in a monolayer on extracellular matrix, and human intestinal lumen containing differentiated chorionic cells and goblet cells is prepared. A 2D organoid for infection / proliferation culture of human diarrhea virus obtained by the step 2 of obtaining a 2D guide in which the epithelial cells constituting the cells have a monolayer structure. |