abstract |
The present invention is a method of suppressing differentiation of stem cells, comprising (1) applying the stem cells to a polymer porous membrane, and (2) culturing and expanding the stem cells, wherein The polymer porous membrane is a three-layered polymer porous membrane having a surface layer A and a surface layer B having a plurality of pores, and a macrovoid layer sandwiched between the surface layer A and the surface layer B. Here, the average pore diameter of the pores present in the surface layer A is smaller than the average pore diameter of the pores present in the surface layer B, and the macrovoid layer is a partition bonded to the surface layers A and B, The method relates to the method, which has a partition wall and a plurality of macrovoids surrounded by the surface layers A and B, and the holes in the surface layers A and B communicate with the macrovoids. |