http://rdf.ncbi.nlm.nih.gov/pubchem/patent/JP-WO2017179375-A1
Outgoing Links
| Predicate | Object |
|---|---|
| classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2502-08 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2502-086 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2502-28 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2533-90 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2523-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2533-52 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N5-069 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-5064 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N5-10 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N5-0618 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-5058 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N5-0622 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-5082 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N5-0697 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N5-02 |
| filingDate | 2017-03-22-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2019-02-28-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | JP-WO2017179375-A1 |
| titleOfInvention | Blood brain barrier in vitro model and method for producing blood brain barrier in vitro model |
| abstract | The present invention aims to provide an in vitro model that reproduces the anatomical structure of the BBB and a method for producing an in vitro model that reproduces the anatomical structure of the BBB, and includes the following (a) to (e): A method for producing a blood-brain barrier in vitro model including a step. (A) culturing conditioned immortalized astrocytes on one side of the porous membrane and conditioned immortalized brain pericytes on the other side of the porous membrane until they become sheets, (b) conditions A step of culturing the immortalized brain microvascular endothelial cells in a culture vessel until they become a sheet, (c) a step of peeling off the condition-immortalized brain microvascular endothelial cells prepared in step (b), and a step (d) ( The step of contacting the sheet of the conditionally immortalized brain microvascular endothelial cell prepared in c) with the sheet of the conditionally immortalized brain pericyte cultured in the step (a), and (e) prepared in the step (d) Co-culturing a cell culture comprising three layers: a sheet of conditioned immortalized brain microvascular endothelial cells, a sheet of conditioned immortalized brain pericyte and a sheet of conditioned immortalized astrocytes |
| priorityDate | 2016-04-15-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 70.