http://rdf.ncbi.nlm.nih.gov/pubchem/patent/JP-WO2017175736-A1
Outgoing Links
Predicate | Object |
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classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N5-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N1-00 |
classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N5-00 |
filingDate | 2017-04-03-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationDate | 2019-02-14-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber | JP-WO2017175736-A1 |
titleOfInvention | Method for preparing membrane vesicles suitable for biophysical analysis |
abstract | (1) a step of ultrafiltration treatment of a sample solution containing membrane vesicles; (2) a step of replacing the sample solution obtained by the step (1) with a buffer solution containing no colloidal flocculant; Thus, a buffer solution containing membrane vesicles is prepared, and (3) a membrane solution including the step of separating the membrane vesicles contained in the buffer solution obtained by the step (2) by density gradient centrifugation. Methods for preparing cell populations are provided. A membrane vesicle population separated and purified from the sample solution, wherein the membrane vesicles contained in the membrane vesicle population are dispersed; and (i) the membrane vesicles contained in the membrane vesicle population (Ii) The membrane vesicles contained in the membrane vesicle population were present in the sample solution before separation and purification. Provided is a membrane vesicle population that maintains its shape and / or (iii) a particle size distribution pattern when the membrane vesicle population is present in the sample solution prior to separation and purification To do. |
priorityDate | 2016-04-04-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 43.