| abstract |
Provided are a cytochrome b-glucose dehydrogenase fusion protein with modified electron transfer characteristics, and a glucose measurement method and measurement kit using the same. A cytochrome b-glucose dehydrogenase fusion protein in which glucose dehydrogenase having homology with SEQ ID NO: 1 or SEQ ID NO: 4 and cytochrome b are linked, and a glucose measurement method, a reagent kit and a sensor using the same. The cytochrome b-glucose dehydrogenase fusion protein of the present invention has a modified electron transfer property and can perform glucose measurement in the presence of a lower concentration of free mediator or in the absence of free mediator, for example, continuous It can be used for glucose measurement. |