| abstract |
There is provided a method for producing pancreatic blasts, comprising a step of culturing PDX1-positive NKX6.1-negative cells in a medium containing KGF, EGF, BMP inhibitor and Akt inhibitor. The culture process may be performed by adhesion culture. PDX1-positive NKX6.1-negative cells can be induced in vitro from pluripotent stem cells, and the present method enables production of pancreatic blasts from pluripotent stem cells. |