| abstract |
It is to produce and / or maintain a naive pluripotent stem cell capable of highly expressing a gene important for maintaining undifferentiated ability, which could not be achieved by a known method for producing pluripotent stem cells. According to the present invention, among the so-called reprogramming factors, six genes (Oct3 / 4, Klf4, c-Myc, Sox2, Nanog and Klf2) are introduced and temporarily expressed, To produce naive pluripotent stem cells capable of maintaining undifferentiated ability by culturing in a medium containing LIF, MEK inhibitor, GSK3 inhibitor, cAMP production promoter, TGF-β inhibitor and PKC inhibitor Therefore, the problem of the present invention can be solved. |