| abstract |
Provided is a sample preparation kit for providing an extremely versatile analytical technique that is not affected by antibody diversity, interspecies differences, matrix, and the like. A sample preparation kit for detecting monoclonal antibodies by high performance liquid chromatography mass spectrometry (LC-MS), comprising a porous material for immobilizing the monoclonal antibody to be measured, and nanoparticles having immobilized protease A reaction vessel for selectively digesting monoclonal antibodies by bringing the porous body and the nanoparticles into contact with each other, and a digestion reaction by the protease introduced into the reaction vessel together with the nanoparticles and the porous body A kit for removing the porous body and the nanoparticles after the digestion reaction, and a filtration membrane for extracting the product of the digestion reaction together with the buffer solution. provide. |