http://rdf.ncbi.nlm.nih.gov/pubchem/patent/JP-WO2016108288-A1
Outgoing Links
Predicate | Object |
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classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A61K35-12 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A61K35-32 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A61K35-34 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A61L27-00 |
classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A61L27-38 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A61P21-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A61P21-04 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A61K35-34 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N5-077 |
filingDate | 2015-12-29-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationDate | 2017-10-05-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber | JP-WO2016108288-A1 |
titleOfInvention | Method for producing skeletal muscle progenitor cells |
abstract | The present invention is a method for producing skeletal muscle progenitor cells from pluripotent stem cells, which comprises the following steps (1) and (2A) or (2B): (1) Step of culturing a pluripotent stem cell in a culture solution containing a TGF-β inhibitor and a GSK3β inhibitor (2A) The cell obtained in the step (1) contains HGF and further contains IGF1 Cells obtained in the step (2B) (1) of culturing in a good culture solution (i) in a culture solution containing a TGF-β inhibitor, a GSK3β inhibitor, IGF1, HGF and bFGF, (ii) in a culture medium containing a TGF-β inhibitor, a GSK3β inhibitor and IGF1, and (iii) A step of sequentially culturing in a culture solution containing a TGF-β inhibitor, IGF1 and HGF is provided. The method further includes the following step (3): (3) A step of culturing the cells obtained in the step (2A) or (2B) in a culture solution containing a TGF-β inhibitor, IGF1 and serum may be included. |
priorityDate | 2014-12-29-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 774.