| abstract |
This is a method for purifying a physiologically active substance including a cation exchange chromatography step and an anion exchange chromatography step after the affinity chromatography step. After the elution of the physiologically active substance in the affinity chromatography step, the buffer of the eluate containing the physiologically active substance is exchanged. In the cation exchange chromatography step, the cation exchange chromatography step and the anion exchange chromatography step are performed in a flow-through manner to purify the physiologically active substance. In the cation exchange chromatography step, a cation having a cation exchange group at a density higher than 30 mmol / L. A method for purifying a physiologically active substance using an exchange carrier. |