| abstract |
The present invention provides a serum-free culture method and serum-free medium for human chondrocytes. An enzyme treatment step for treating a tissue containing human chondrocytes with a proteolytic enzyme, an inhibitor treatment step for treating the tissue with an inhibitor of the proteolytic enzyme after the enzyme treatment step, and an inhibitor A serum-free culture method of chondrocytes, comprising a culture step of culturing the tissue in a serum-free medium containing ITS, FGF2, and hydrocortisone after the treatment step, either or both of cultogenin and SAG. A serum-free medium for chondrocyte culture containing cultogenin or SAG or both, ITS, FGF2, and hydrocortisone. [Selection] Figure 1 |