| abstract |
Provided are an amadoriase that is not easily affected by oxygen concentration, and a method and a reagent kit for measuring HbA1c using the same. Substituting one or more amino acid residues at a position corresponding to a position selected from the group consisting of positions 280, 267, 269, 54 and 241 of amadoriase derived from the genus Coniochaeta And a method for measuring HbA1c using the same, a reagent kit and a sensor. The modified amadoriase of the present invention has reduced oxidase activity and improved dehydrogenase activity, enables the use of an electron mediator, reduces the influence of oxygen concentration, and can measure HbA1c with high sensitivity. |