abstract |
A method for measuring at least one of a kinase positive reaction substrate, its phosphate, and a pre-induction substance induced therein, comprising at least a kinase, a first nucleotide coenzyme of the kinase, and a first nucleotide coenzyme Contacting a sample with a second nucleotide coenzyme having a different nucleoside moiety and reacting an enzyme cycling reaction, and at least one of the first nucleotide coenzyme and its variant, and the second nucleotide coenzyme and its variant A step of detecting a signal corresponding to any amount of change, and (3) a step of calculating the amount of the positive reaction substrate of the kinase and / or its phosphate contained in the sample based on the amount of change of the detected signal. And a measurement method including: |