http://rdf.ncbi.nlm.nih.gov/pubchem/patent/JP-WO2016010082-A1
Outgoing Links
| Predicate | Object |
|---|---|
| classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K2319-42 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2533-52 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K2319-21 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K2319-43 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-09 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N5-0696 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K14-78 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N5-071 |
| filingDate | 2015-07-15-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2017-06-22-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | JP-WO2016010082-A1 |
| titleOfInvention | Method for enhancing cell culture substrate activity of laminin fragment |
| abstract | In a cell culture method using a cell culture device coated with a laminin fragment, a novel technique is provided that enables cell culture equivalent to the case of coating at the recommended concentration even when a coating concentration lower than the recommended coating concentration is used. . The present invention relates to a method for enhancing the activity of a laminin fragment having an integrin-binding activity or a variant thereof on cultured mammalian cells, comprising a laminin fragment or a variant thereof and a protein other than laminin or a fragment thereof. In contact with the culture surface of the cell culture device and coating the culture surface with the laminin fragment or variant thereof, wherein the coating solution comprises an amount of laminin fragment or modification thereof that results in a coating concentration of less than 0.5 μg / cm 2. It is an activity enhancing method characterized in that it contains a body and the other protein concentration is 50 μg / mL or more. |
| priorityDate | 2014-07-16-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 995.