http://rdf.ncbi.nlm.nih.gov/pubchem/patent/JP-WO2015041219-A1
Outgoing Links
| Predicate | Object |
|---|---|
| classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K2319-80 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K14-4702 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K14-395 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K14-37 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6897 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-09 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N5-10 |
| filingDate | 2014-09-17-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2017-03-02-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | JP-WO2015041219-A1 |
| titleOfInvention | Optogenetic control of stem cell proliferation and differentiation |
| abstract | The present invention uses an optogenetic gene expression control system to control the expression of neuronal differentiation determinants by light irradiation, thereby proliferating and maintaining neural progenitor (NPC) cells, or from neural stem cells to the nervous system. A method of controlling whether to induce differentiation into a cell is provided. In the present invention, neural differentiation factors such as Ascl1, Hes1, and Olig2 are periodically oscillating in NPC, but the discovery that any one of them is dominant during fate determination, This is based on the construction of a gene expression system that can periodically oscillate the expression of a target gene. That is, the present invention relates to a nucleic acid encoding a transcriptional activator (GAL4-Vivid-p65 fusion protein; GAVPO) that is activated (dimerized) in response to blue light and a cis-sequence that binds the transcriptional activator. A DNA encoding a target neuronal differentiation determinant and a nucleic acid linked to 3'-UTR that destabilizes mRNA are introduced into an NPC downstream of a promoter containing, and blue light is applied to the NPC for a short period of time by changing the period. By irradiating, a method of oscillating a target factor to maintain and amplify NPC, or inducing a continuous expression of the target factor to induce differentiation into a nervous system cell destined by the factor is provided. |
| priorityDate | 2013-09-18-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 487.