http://rdf.ncbi.nlm.nih.gov/pubchem/patent/JP-WO2014156513-A1
Outgoing Links
| Predicate | Object |
|---|---|
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6816 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-68 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-09 |
| filingDate | 2014-03-04-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2017-02-16-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | JP-WO2014156513-A1 |
| titleOfInvention | Mutation detection method |
| abstract | A DNA sample that may have a mutation, a first query probe having a first identification sequence for identifying a mutation, and a second query having a second identification sequence for identifying a region adjacent to the mutation A probe is brought into contact with each other, a DNA ligase reaction is performed, and the first identification sequence and the second identification sequence are hybridized specifically with the probe detection sequence corresponding to the type of mutation. A ligation product each having a sequence for labeling and a labeling sequence for imparting a label, the ligation product, a first primer having a sequence complementary to the hybridization sequence, and the labeling sequence An amplification product comprising the labeling sequence and the hybridizing sequence by a DNA polymerase amplification reaction in contact with a second primer having Obtaining a hybridized product by contacting the probe on the solid phase carrier with the amplification product, and the total concentration of the second primer is equal to the total concentration of the first primer. 0.5 times or more and less than 12.5 times. |
| priorityDate | 2013-03-29-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Total number of triples: 10.