http://rdf.ncbi.nlm.nih.gov/pubchem/patent/JP-WO2014126260-A1
Outgoing Links
| Predicate | Object |
|---|---|
| classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K2319-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K2319-50 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K2319-70 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K1-36 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K1-34 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K14-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K1-30 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K14-32 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K14-34 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K14-635 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K14-62 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K14-815 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12P21-02 |
| filingDate | 2014-02-18-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2017-02-02-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | JP-WO2014126260-A1 |
| titleOfInvention | Production method by protein precipitation |
| abstract | The present invention provides a method for producing a target protein as a fusion protein with a high recovery rate. The present invention is a method for producing a fusion protein of a protein having a self-assembling ability and a target protein, the following steps (1) to (4): (1) preparing a solution containing the fusion protein; (2) The pH of the solution obtained in step (1) is calculated by the following formula: Recovery rate (%) = [Amount of fusion protein in solution obtained in step (4) / {Amount of fusion protein in solution obtained in step (4) + Fusion in solution after solid separation in step (3) Protein amount}] × 100 A step of adjusting the pH calculated to be 10% or higher, (3) separating the solid from the solution obtained in step (2), and (4) Fusion including the step of dissolving the solid separated in step (3) in a solution having a pH of 0.1 or higher and 12 or lower than the pH of the solution obtained in step (2) A method for producing a protein. |
| priorityDate | 2013-02-18-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 534.