abstract |
To provide a practical and highly productive c-di-GMP enzyme synthesis method. Diguanylate cyclase having the following physicochemical properties (A) to (F) is used. (A) Action: Catalyze the following reaction. 2GTP → c-di-GMP, (B) molecular weight: 19800 ± 2000, (C) optimum pH: 7.3 to 9.4, (D) optimum temperature: 35 to 60 ° C, (E) thermal stability: 50 ° C, (F) GGDEF domain having a residual activity of 90% or more when heated at pH 7.8 for 60 minutes, and does not have KXXD amino acid sequence at i-site. |