| abstract |
It is an object of the present invention to develop and provide a method for efficiently producing a nucleic acid aptamer, particularly a DNA aptamer, having higher specificity and binding property to a target substance than a nucleic acid aptamer obtained by a conventional method. Provided is a nucleic acid aptamer comprising a natural nucleotide and a non-natural nucleotide having an artificial base capable of artificial base pairing, and capable of transcription or replication. Also provided is a method for determining the base sequence of a non-natural nucleotide-containing single-stranded nucleic acid molecule selected from a single-stranded nucleic acid library. |