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filingDate 2011-12-22-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationDate 2015-02-23-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationNumber JP-WO2012168991-A1
titleOfInvention Method for detecting antigen
abstract The present invention relates to a method for detecting an antigen using an antibody and an enzyme. Specifically, the present invention provides a method for detecting an antigen without using a labeled antibody. This method includes a step of immersing particles in a first buffer that is expected to contain an antigen, and an antibody and multi-copper oxidase CueO are immobilized on the surface of the particles, and the antibody is specific to the antigen. Reacts energetically. This method further includes a step of collecting the obtained particles, a step of mixing the collected particles, a redox indicator (reduced form), and a second buffer to prepare a reaction solution, and the obtained reaction solution A step of measuring the activity of the multi-copper oxidase CueO contained in the sample by an absorbance measurement method, and a step of determining that the first buffer contains the antigen based on the step of measuring the activity.
priorityDate 2011-06-06-04:00^^<http://www.w3.org/2001/XMLSchema#date>
type http://data.epo.org/linked-data/def/patent/Publication

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