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filingDate 2011-12-27-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationDate 2014-06-05-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationNumber JP-WO2012091056-A1
titleOfInvention Test method for target cells in blood, target cell search device, and biochip
abstract In order to perform non-invasive and safe prenatal diagnosis of the fetus quickly and accurately, fetal-derived target cells that are present in very small amounts in the collected maternal blood are appropriately concentrated, or abnormalities present in the blood of infants or adults The target cells are appropriately concentrated, searched on the flat plate or in the flow path of the biochip in a short time with high accuracy, and the target cells are reliably recovered after identifying their positions, and the chromosomes in the cell nucleus Provided is a simple and efficient method for examining target cells in blood for accurately analyzing DNA and RNA. The method for testing target cells in blood involves performing a target dictionary creation step and a non-target dictionary creation step in advance, and then performing a sample acquisition step, a sample labeling step, an optical label expression step, a focus on the blood to be tested. Control process, color image acquisition process, binarization process, contraction and expansion process, primary candidate search process, secondary candidate search process, tertiary candidate search process, target cell identification process, target cell recovery process, and target cell inspection The target cells in the blood are examined by performing each of the steps.
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