| abstract |
An inhibitor of activation of an immune response mediated by HMGB protein, comprising one or more compounds selected from the group consisting of phosphorothioate oligonucleotides and derivatives thereof, and in the presence and absence of a test substance, The mixing step of mixing the HMGB protein and the labeled nucleic acid, the quantifying step of quantifying the HMGB protein bound to the labeled nucleic acid, and the amount of the HMGB protein bound to the labeled nucleic acid in the presence of the test substance When the amount of the HMGB protein bound to the labeled nucleic acid in the presence is less than the amount of the test substance, the test substance is determined to be an inhibitor of activation of the immune response mediated by the HMGB protein, and the presence of the test substance The amount of HMGB protein bound to the labeled nucleic acid in step 1 is greater than the amount of HMGB protein bound to the labeled nucleic acid in the absence of the test substance. A determination step for determining that the test substance is an accelerator for activation of an immune response mediated by HMGB protein, and an inhibitor of activation of an immune response mediated by HMGB protein, Alternatively, a method for screening an accelerator is provided. |