http://rdf.ncbi.nlm.nih.gov/pubchem/patent/JP-WO2012035705-A1
Outgoing Links
| Predicate | Object |
|---|---|
| classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N2800-52 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N21-6456 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N21-6486 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N21-6428 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-94 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-582 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-6854 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N21-64 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N21-78 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-536 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-574 |
| filingDate | 2011-08-26-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2014-01-20-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | JP-WO2012035705-A1 |
| titleOfInvention | Method for determining the effectiveness of pharmaceuticals containing antibodies as components |
| abstract | By quantitatively staining a living tissue, it is possible to accurately quantify the antibody that is an active ingredient of an antibody drug such as trastuzumab and the protein recognized by the antibody for targeting the active ingredient to the target site. The present invention provides a method for determining the therapeutic effectiveness of a pharmaceutical comprising an antibody as a component. A step of labeling an antibody in a pharmaceutical containing an antibody as a component with a fluorescent substance and bringing the fluorescently labeled antibody into contact with a tissue sample; a step of irradiating a tissue site in contact with the antibody with excitation light to acquire a fluorescence image; Obtaining a self-fluorescent image in a short wavelength side or a long wavelength side near a fluorescent wavelength obtaining region emitted from the fluorescent material in the same field of view and in the same focus as the fluorescent image; from the fluorescent luminance of the fluorescent image; Performing image processing excluding the fluorescence brightness of the autofluorescence image and obtaining a corrected fluorescence image; counting the number of cells in the tissue site in contact with the antibody; measuring the average fluorescence brightness of one fluorescent particle; 1 Using a tissue staining method including a step of calculating the number of fluorescent particles per cell, the effectiveness of a drug containing an antibody as a component is determined. |
| priorityDate | 2010-09-17-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 366.