http://rdf.ncbi.nlm.nih.gov/pubchem/patent/JP-WO2011090154-A1
Outgoing Links
| Predicate | Object |
|---|---|
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6858 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6827 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-09 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-68 |
| filingDate | 2011-01-21-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2013-05-23-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | JP-WO2011090154-A1 |
| titleOfInvention | Target sequence amplification method, polymorphism detection method and reagent used therefor |
| abstract | Aim to provide an amplification method that suppresses amplification due to erroneous primer annealing Primers X1 and X2 are used in amplification of a target sequence containing a target site exhibiting a polymorphism. X1 has a sequence A1 ′ and a sequence E1, and A1 ′ is a sequence complementary to the partial sequence A1 in the template nucleic acid, and the 3 ′ region thereof includes the first of the target site in the 5 ′ region of A1. A base x1 ′ complementary to the base x1, and E1 is a sequence that is non-complementary to the partial sequence B1 adjacent to the 3 ′ end of A1 in the template nucleic acid, and binds to the 5 ′ end of A1 ′ Primer. X2 has the sequence A2 ′, A2 ′ is a sequence complementary to the partial sequence A2 in the template nucleic acid, and in the 3 ′ region thereof, the second base x2 of the target site in the 5 ′ region of A2 A primer having a complementary base x2 ′ is used. X1 and X2 both have a base complementary to the target site in the 3 ′ region. These primers can prevent erroneous amplification of the target sequence having the second base x2 when the target site is only the template having the first base x1. Thereby, a false positive can be suppressed about the polymorphism of the 2nd base x2. |
| priorityDate | 2010-01-21-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 61.