abstract |
A novel enzyme that stereoselectively reduces an imine derivative was isolated and purified, and a polynucleotide encoding the enzyme was cloned. By reacting the imine derivative with a culture of a microorganism having the ability to reduce the compound in a stereoselective manner, a microbial cell or a treated product thereof, or an imine reductase, and recovering the optically active amine derivative produced, An optically active amine derivative is produced. According to the present invention, for example, an optically active compound represented by the following formula (IV) can be produced. Formula (IV) [Chemical 1] (In the formula, R represents an alkyl group having 1 to 3 carbon atoms, and n represents an integer of 1 to 4) |