http://rdf.ncbi.nlm.nih.gov/pubchem/patent/JP-WO2007060764-A1
Outgoing Links
Predicate | Object |
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classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2800-30 |
classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-63 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-64 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-85 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6867 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6844 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12P19-34 |
classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12P21-02 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-09 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N5-10 |
filingDate | 2006-07-18-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationDate | 2009-05-07-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber | JP-WO2007060764-A1 |
titleOfInvention | Gene amplification method |
abstract | PROBLEM TO BE SOLVED: To provide a double-stranded DNA specially constructed for amplifying a gene at high speed, a gene amplification method using the same, and a protein synthesis method. [Solution] The amplification system of the present invention utilized a site-specific recombination enzyme such as a Cre-lox system and its target sequence in order to efficiently induce a replication reaction called double rolling-circle replication (DRCR). A replication unit having the structure shown in FIG. 2 (a) is constructed in a cell such as an animal cell, and is generated by a site-specific recombination enzyme (Cre) when the replication fork advances between a pair of target sequences (lox sequences). Recombination is used to induce DRCR. [Selection] Figure 2 |
priorityDate | 2005-11-24-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 1102.