http://rdf.ncbi.nlm.nih.gov/pubchem/patent/JP-WO2006137550-A1

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filingDate 2006-06-19-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationDate 2009-01-22-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationNumber JP-WO2006137550-A1
titleOfInvention Cancer diagnosis method and lung cancer diagnosis method
abstract In the early stages of lung cancer, evidence of the presence of cancer cells can be detected in the blood, and if the cancer diagnosis is cancer, it may be judged negative even though it is a lung cancer patient. Provided are a cancer diagnosis method and a lung cancer diagnosis method using such a cancer diagnosis method, which are significantly reduced in cases where the patient is judged to be positive even though the patient is not a patient. From a patient's body fluid, obtaining a sample containing only RNA as a somatic / cancer cell component, PCR using a reverse transcriptase reaction and a fluorescent dye to generate cDNA from a sample containing only RNA using reverse transcriptase A first cancer comprising: a step of detecting the presence of EGFR mRNA using a primer set, and quantitatively measuring a PCR product amplified by PCR using a fluorescent dye combined with the PCR product. A diagnostic method, a step of obtaining a sample containing only RNA as a somatic cell / cancer cell component from the body fluid of the patient, a reverse transcriptase reaction and fluorescence for generating cDNA from a sample containing only RNA by reverse transcriptase PCR using a dye is performed using a primer set that detects the presence of hTERT mRNA, and the PCR product amplified by the PCR is converted to the PCR A second cancer diagnostic method comprising a step of quantitatively measuring using a fluorescent dye bound to the product, and the results of both the first cancer diagnostic method and the second cancer diagnostic method are positive If determined, it is determined as cancer.
priorityDate 2005-06-20-04:00^^<http://www.w3.org/2001/XMLSchema#date>
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