abstract |
An object of the present invention is to provide a stem cell that can be used in a method for regenerative treatment, and a technique for performing regenerative treatment using the cell. A cell suspension was prepared by enzymatically treating the collected cardiac tissue pieces, and using the cell suspension, (1) separation of cells by a density gradient method, (2) fibroblast growth factor and epithelium. Pluripotent stem cells are obtained by suspension culture in a medium containing a cell growth factor and (3) selective separation of cells forming suspension-like spheres, which are used for regenerative treatment. |