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filingDate 2004-03-15-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationDate 2006-07-27-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationNumber JP-WO2004087761-A1
titleOfInvention Purification of human monoclonal antibodies and human polyclonal antibodies
abstract Provided is a method for separating and purifying human monoclonal antibodies and human polyclonal antibodies mainly used for therapeutic applications, and sometimes used for diagnostic and reagent applications, and comprises at least the following steps from a mixture containing antibodies. A method for purifying an antibody; (1) a step of purifying by anion exchange chromatography; and (2) a step of purifying by cation exchange chromatography following (1); and Provided is a method for separating the human antibody and the antibody of the artiodactyla from a mixture containing the antibody by pH gradient using protein A affinity chromatography.
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