http://rdf.ncbi.nlm.nih.gov/pubchem/patent/JP-WO2004078965-A1
Outgoing Links
Predicate | Object |
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classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12P21-02 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-70 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K14-43531 |
classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K14-435 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12P21-02 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12P21-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-09 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A61K39-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-70 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K14-195 |
filingDate | 2004-02-20-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationDate | 2006-06-08-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber | JP-WO2004078965-A1 |
titleOfInvention | Method for producing heterologous proteins in E. coli |
abstract | Provided are a heterologous protein containing no expression inducer and a method for producing the same. Recombinant Escherichia coli expressing a heterologous protein under the control of a promoter capable of inducing expression by a temperature shift is arbitrarily grown at low temperature, and then cultured at high temperature in the absence of an expression inducer. A method for producing a heterologous protein comprising a step of expressing a protein, or a step of culturing the above-mentioned Escherichia coli at a high temperature to simultaneously proliferate the cells and express the heterologous protein, and a heterologous protein containing no expression inducer obtained by the method. Specific examples of such heterologous proteins are mite major allergens, secreted macrophage toxins derived from porcine pleural pneumococcus, and swine erysipelas infection protective antigen. |
priorityDate | 2003-03-05-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 158.