abstract |
At least one of the amino acids constituting the protein and having the following labeling pattern: (A) one or more methylene groups are deuterated, leaving at least one of the methylene hydrogens; (b) in the prochiral gem-methyl group, the hydrogen of one methyl group is completely (C) In the prochiral methyl group, which is deuterated, the hydrogen of the methyl group is partially deuterated. (D) The methyl group is deuterated, leaving one hydrogen, and the aromatic ring hydrogen is partially There is provided a stable isotope-labeled amino acid characterized by having at least one of deuterated. Using this stable isotope-labeled amino acid, protein deuterium substitution can be achieved without compromising the NMR sensitivity of the residual hydrogen nuclei, and rapid and reliable analysis of NMR spectra of high molecular weight proteins exceeding conventional limits. A highly accurate determination of the three-dimensional structure can be achieved simultaneously. |