http://rdf.ncbi.nlm.nih.gov/pubchem/patent/JP-WO2002046395-A1
Outgoing Links
| Predicate | Object |
|---|---|
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K1-13 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12P21-02 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12P21-02 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12P21-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K14-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K1-14 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K1-13 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-09 |
| filingDate | 2001-12-07-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2004-04-08-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | JP-WO2002046395-A1 |
| titleOfInvention | C-terminal modified protein and method for producing the same, modifying agent and translation template used for producing C-terminal modified protein, and method for detecting protein interaction using C-terminal modified protein |
| abstract | In a protein translation system, a C-terminal modifier for a protein, comprising: an acceptor moiety having a group capable of binding to a protein by a transpeptidation reaction; and a modifying moiety containing a modifying substance bound to the acceptor moiety via a nucleotide linker An ORF region encoding a protein, a 5 'untranslated region containing a transcription promoter and a translation enhancer located 5' to the ORF region, and a poly A sequence located 3 'to the ORF region. A translation template containing a 3′-terminal region containing the above is expressed in a translation system to perform protein synthesis, and the synthesized protein is purified. As a result, the yield of the modified protein in the C-terminal modification method of the protein is greatly improved, and the detection of protein interaction by various intermolecular interaction assays is realized at an improved level. |
| priorityDate | 2000-12-07-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 276.