http://rdf.ncbi.nlm.nih.gov/pubchem/patent/JP-S6480286-A
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_27176f5bbbfd702de498d84df8ff3ae5 |
| classificationIPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12R1-01 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N9-20 |
| filingDate | 1987-09-22-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_6181e3188eb84d7878ec08595106d1e8 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_4742ae2f412ed8864be8d034293660b0 |
| publicationDate | 1989-03-27-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | JP-S6480286-A |
| titleOfInvention | Method for purifying enzyme |
| abstract | PURPOSE:To carry out fractionation into 1,3-diglyceride lipase and 2- monoglyceride lipase, by subjecting a lipase obtained by cultivating Candida.cylindraceae to treatment using column chromatography. CONSTITUTION:An ion exchange gel, such as DEAE-Sephadex(R) or SP- Sephadex(R), or gel for gel filtration, such as Sephadex(R) G-150 or G-100, or both are used as a carrier, treated with MacIlvaine buffer solution (0.01mol. citric acid and 0.02mol. disodium phosphate; pH 3.8), etc., filled in a column having 1-10cm diameter, 50-200cm length, etc., and used for column chromatography. A lipase obtained by cultivating Candida.cylindraceae, such as lipase OF, is treated by the above-mentioned column chromatography and fractionated into 1,3-diglyceride lipase and 2-monoglyceride lipase. |
| isCitedBy | http://rdf.ncbi.nlm.nih.gov/pubchem/patent/KR-100592871-B1 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/JP-2016037556-A |
| priorityDate | 1987-09-22-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 61.