http://rdf.ncbi.nlm.nih.gov/pubchem/patent/JP-S6475963-A
Outgoing Links
Predicate | Object |
---|---|
assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_2b30aaab6b2cd23887e078e5c256e027 |
classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-12 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N31-22 |
filingDate | 1987-09-18-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_4dc915c77afa0faf24736cf578c0ecd8 |
publicationDate | 1989-03-22-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber | JP-S6475963-A |
titleOfInvention | Freshness testing paper |
abstract | PURPOSE:To secure the reproducibility and stability of a developed color, by impregnating filter paper with nucleotide phosphorylase, xanthine oxidase, peroxidase, 2,7-diaminofluolein and o-tolidine. CONSTITUTION:Freshness testing paper is prepared by impregnating filter paper with nucleotide phosphorylase, xanthine oxidase, peroxidase, 2,7-diaminofluolein and o-tolidine. As the filter paper, filter paper for a chromatograph is pref. used. For example, the freshness testing paper is prepared by a method wherein water is added to 5ml of a phosphate buffer solution with pH of about 6-8, about 0.01-0.05ml of 2,7-diaminofluolein, about 0.01-0.05ml of o-tolidine, about 5-15 enzymatic unit of xanthine oxidase, about 10-30 enzymatic unit of nucleo tide phosphorylase, about 0.5-2g of sucrose (enzyme stabilizer) and 0.2ml of an about 0.5-2% aqueous mercaptoethanol (enzyme oxidation inhibitor) solution to adjust the whole to 15ml and this enzyne-dye solution is absorbed by the filter paper and the impregnated one is freeze-dried under vacuum. |
isCitedBy | http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-109633108-A |
priorityDate | 1987-09-18-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 40.