| Predicate |
Object |
| assignee |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_02ff914937eb6ceaee5ca69cc56cbfa7 |
| classificationCPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K7-18 |
| classificationIPCAdditional |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12R1-19 |
| classificationIPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N1-21
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K7-18
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12P21-02
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K7-06
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K1-12
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K19-00
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-09 |
| filingDate |
1987-09-09-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_c1a9f6de0609e39f8e782aa70b7bfaf4
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_d90c311f5e4f29c05d85e15244787fa9
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_ec37ad3bebf177af2ef04ba089f3b48e
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_6c5f660d2af75023e5e806cce9a9abe1 |
| publicationDate |
1989-03-16-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber |
JP-S6471497-A |
| titleOfInvention |
Production of bradykinin |
| abstract |
PURPOSE:To obtain bradykinin having hypotensive action, by separating bacterial cell from E.coli C600 strain containing pBLAK1, treating the cell with cyanogen bromide and separating the objective compound by high-performance liquid chromatography. CONSTITUTION:E.coli C600 strain containing recombinant plasmid pBLAK1 is cultured in a medium, the bacterial cells are collected after cultivation and centrifuged to obtain a protein composed of dihydrofolic acid reductase-reductase fused to bradykinin. The protein is mixed and treated with cyanogen bromide and subjected to high-performance liquid chromatography to obtain bradykinin useful in the fields of medical and pharmaceutical industries, etc. |
| priorityDate |
1987-09-09-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type |
http://data.epo.org/linked-data/def/patent/Publication |