http://rdf.ncbi.nlm.nih.gov/pubchem/patent/JP-S6460380-A
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_fc6220b93fae4be14ada49e4ecc1860b |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N9-96 |
| filingDate | 1987-08-31-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_67d0ecb04245b39faf3452a438273597 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_f76121f118515eb1f077e773212b3472 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_86451d41756b6e02cfc07b47cbdfac8d |
| publicationDate | 1989-03-07-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | JP-S6460380-A |
| titleOfInvention | Stabilization of enzyme |
| abstract | PURPOSE:To stably keep the enzymatic activity of alpha-glycosidase over a long period even in the state of aqueous solution, by bonding alpha-glycosidase with a water-soluble polysaccharide by covalent bond. CONSTITUTION:An alpha-glycosidase is bonded to a water-soluble polysaccharide through a covalent bond. The alpha-glycosidase is an enzyme catalyzing the reaction to hydrolyze the non-reducing terminal alpha-1,4-glycoside bond of a sugar chain and produce alpha-glucose and is useful especially as a conjugated enzyme for the determination of alpha-amylase activity in a biospecimen. The water-soluble polysaccharide is e.g. dextran, dextran sulfate, pullulan, soluble starch or chondroitin sulfate and has a molecular weight of preferably 5,000-500,000. The covalent bond can be formed e.g. by oxidizing the water-soluble polysaccharide, dialyzing the product with a buffer solution to form an aldehyde compound, reacting with an alpha-glycosidase in a buffer solution and dialyzing the reaction product. |
| isCitedBy | http://rdf.ncbi.nlm.nih.gov/pubchem/patent/JP-2009028034-A http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-5391483-A |
| priorityDate | 1987-08-31-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 20.