abstract |
PURPOSE:To selectively assimilate only L-aspartic acid and readily obtain D- aspartic acid, by cultivating a specific microorganism capable of assimilating only the L-aspartic acid in a culture medium containing DL-aspartic acid. CONSTITUTION:A microorganism, belonging to the genus Microbacterium, Bacterium, Escherichia, Serratia, Alcaligenes, Flavobacterium, Klebsiella, Providencia, Pichia, Candida, Cryptococcus, etc., and having selective assimilating ability to assimilate only L-aspartic acid without assimilating D-aspartic acid is cultivated in a culture medium containing DL-aspartic acid as a carbon and nitrogen sources and the resultant culture fluid is then subjected to centrifugation to remove the microbial cells therefrom and afford the aimed D-aspartic acid. |